NClassG+: A classifier for non-classically secreted Gram-positive bacterial proteins

Gpos-PLoc: an ensemble classifier for predicting subcellular localization of Gram-positive bacterial proteins.

A statistical analysis indicated that, of the 35,016 Gram-positive bacterial proteins from the recent Swiss-Prot database, approximately 57% of these entries are without subcellular location annotations. In the gene ontology database, the corresponding percentage is approximately 67%, meaning the percentage of proteins without subcellular component annotations is even higher. With the avalanche...

Subcellular localization for Gram positive and Gram negative bacterial proteins using linear interpolation smoothing model.

Protein subcellular localization is an important topic in proteomics since it is related to a protein׳s overall function, helps in the understanding of metabolic pathways, and in drug design and discovery. In this paper, a basic approximation technique from natural language processing called the linear interpolation smoothing model is applied for predicting protein subcellular localizations. Th...

Synthesis, characterization and antimicrobial properties of CuO nanoparticles against gram-positive and gram-negative bacterial strains

Nano structured materials have wide range of applications due to their interesting size-dependent chemical and physical properties compared to particles of size in the range of micrometer. Copper oxide nano materials are of interest on account of their potential uses in many technological fields. In this study  CuO nanoparticles were synthesized via simple sol gel method using basic CuSO4<...

Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive microorganisms.

We describe the construction of TnFuZ, a genetic tool for the discovery and mutagenesis of proteins exported from gram-positive bacteria. This tool combines a transposable element (Tn4001) of broad host range in gram-positive bacteria and an alkaline phosphatase gene (phoZ) derived from a gram-positive bacterium that has been modified by removal of the region encoding its export signal. Mutagen...

Bacterial Infections: Gram-Positive Cocci